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F-Actin抗体
ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500
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not yet tested in oth 【详细说明】
英文名称 F-Actin
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中文名称 纤维状肌动蛋白抗体
别 名 f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1.
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F-Actin抗体
说 明 书 0.1ml 0.2ml
研究领域 细胞骨架
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Sheep,
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500
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（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
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免 疫 原 KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit
亚 型 IgG
纯化方法 affinity purified by Protein A
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储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
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保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
F-Actin抗体
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产品介绍 background:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells .G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other.
Function:
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Subunit:
Heterodimer of an alpha and a beta subunit. Interacts with S100A (By similarity). Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts with S100B.
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Subcellular Location:
Cytoplasm, cytoskeleton.
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Tissue Specificity:
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Similarity:
Belongs to the F-actin-capping protein alpha subunit family.
Gene ID:
60
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Database links:
important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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