广州市硕恒生物科技有限公司是广州知名的试剂供应商,产品涉及分子实验,蛋白组学,细胞培养,微生物培养,植物组培,生化实验,耗材,仪器等,主要代理的品牌有:Sigma,Invitrogen (Gibco)、abcam 、Merckmillipore、Roche ,Qaigen、GE、BD、NEB,南京建成、R D、Peprotech、CST、Santa Cruz 、北京康为世纪、Pharmacia、Thermo, Wako、Whatman、CeneCopoeia、Clontech、Omega,Promega,KPL、天恩泽,Amresco等。耗材类主要有:NUNC,Axygen、Corning。
品牌:Sciencell是否是肿瘤细胞:0运输方式:干冰年限:长期数量:大量规格:5 x 10^5 cells/vial
人脐静脉内皮细胞说明:
货号:8000
血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的细胞进行体外研究,人脐静脉和动脉内皮细胞是这类研究中zui常用的细胞类型。除了普通的内皮细胞特征例如“鹅卵石状”,VIII因子阳性着色,能摄取乙酰基化低密度脂蛋白,人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。 人脐静脉内皮细胞(HUVEC)提取于人脐带静脉组织,原代冻存。每管含有细胞数 5×105 cells/ml,此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基: (ECM, Cat. No. 1001)
The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition to mediators that influence the adhesion and aggregation of blood plaets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone. Many of the endothelial processes can be studied in vitro using cultured cells, and human umbilical vein endothelial cells (HUVEC) are the most commonly used cell type for such studies. Except for the common endothelial cell features such as "cobblestone" morphology, positive staining for Factors VIII and the ability to take up acetylated low-density lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin [3] and VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are cryopreserved at passage one and delivered frozen. Each vial contains 5 x 105 cells in 1 ml volume. HUVEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Reference
1. Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In "Human Cell Culture Protocols" (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243:1`160-1165.
4. Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211.
Sciencell产品目录:
胎盘 细胞系统中文名货号简称英文名称人羊膜上皮细胞7110HAEpiCHuman Amniotic Epithelial Cells人长茸毛滋养层7120HVTHuman Villous Trophoblasts人长茸毛间叶成纤维细胞7130HVMFHuman Villous Mesenchymal Fibroblasts 脂肪细胞系统中文名货号简称英文名称人脂肪微血管内皮细胞7200HAMECHuman Adipose Microvascular Endothelial Cells人脂肪前体细胞-内脏7210HPA-vHuman Preadipocytes-visceral人脂肪前体细胞-皮下7220HPA-sHuman Preadipocytes-subcutaneous 卵巢细胞系统中文名货号简称英文名称人卵巢微血管内皮细胞7300HOMECHuman Ovarian Microvascular Endothelial Cells人卵巢表面上皮细胞7310HOSEpiCHuman Ovarian Surface Epithelial Cells人卵巢成纤维细胞7330HOFHuman Ovarian Fibroblasts 间充干细胞系统中文名货号简称英文名称人羊膜间充质干细胞7140HAmMSCHuman Amniotic Mesenchymal Stromal Cells人绒毛膜间充质干细胞7150HCMSCHuman Chorionic Mesenchymal Stromal Cells人骨髓间充质干细胞7500HMSC-bmHuman Bone Marrow-derived Mesenchymal Stem Cells人脂肪间充质干细胞7510HMSC-adHuman Adipose-derived Mesenchymal Stem Cells人肝间充质干细胞7520HMSC-heHuman Liver-derived Mesenchymal Stem Cells人脐间充质干细胞7530HUMSCHuman Umbilical Mesenchymal Stem Cells人肺间充质干细胞7540HPMSCHuman Pulmonary Mesenchymal Stem Cells人脊椎间充质干细胞7550HVMSCHuman Vertebral Mesenchymal Stem Cells 乳腺细胞系统中文名货号简称英文名称人乳腺微血管内皮细胞7600HMMECHuman Mammary Microvascular Endothelial Cells人乳腺上皮细胞7610HMEpiCHuman Mammary Epithelial Cells人乳腺成纤维细胞7630HMFHuman Mammary Fibroblasts 干细胞诱导细胞中文名货号简称英文名称人iPS诱导心肌细胞6240HPSC-CCHPSC-derived Cardiomyocyte Cells 脐带细胞系统中文名货号简称英文名称人脐静脉内皮细胞8000HUVECHuman Umbilical Vein Endothelial Cells人脐动脉内皮细胞8010HUAECHuman Umbilical Artery Endothelial Cells人脐静脉平滑肌细胞8020HUVSMCHuman Umbilical Vein Smooth Muscle Cells人脐动脉平滑肌细胞8030HUASMCHuman Umbillical Artery Smooth Muscle Cells 大鼠细胞系统中文名货号简称英文名称大鼠脑微血管内皮细胞R1000RBMECRat Brain Microvascular Endothelial Cells大鼠垂体细胞R1200RPCRat Pituitary Cells大鼠脑膜细胞R1400RMCRat Meningeal Cells大鼠脑脊神经元R1510RN-rRat Neurons-raphe大鼠皮质神经元R1520RN-cRat Neurons-cortical大鼠颗粒细胞R1530RGCRat Granule Cells大鼠神经元- 海马R1540RN-hRat Neurons-hippocampal大鼠黑质神经元R1550RN-snRat Neurons-substantia nigra大鼠纹状体神经元R1560RN-sRat Neurons-striatal大鼠腹脊髓神经元R1570RN-vscRat Neurons-ventral spinal cord大鼠背脊髓神经元R1580RN-dscRat Neurons-dorsal spinal cord大鼠背脊髓神经元R1590RN-scRat Neurons-spinal cord大鼠少突胶质前体细胞R1600ROPCRat Oligodendrocyte Precursor Cells大鼠雪旺细胞R1700RSCRat Schwann Cells大鼠神经束膜成纤维细胞R1710RPNFRat Perineurial Fibroblasts大鼠星形胶质细胞R1800RARat Astrocytes大鼠星形胶质细胞- 小脑R1810RA-cRat Astrocytes-cerebellar大鼠星形胶质细胞- 海马R1820RA-hRat Astrocytes-hippocampal大鼠小神经胶质细胞R1900RMRat Microglia大鼠巨噬细胞R1920RMaRat Macrophage大鼠表皮角质形成细胞-新生大鼠R2100REK-nRat Epidermal Keratinocytes-neonate大鼠真皮成纤维细胞R2300RDF-nRat Dermal Fibroblasts大鼠真皮成纤维细胞- 成人R2320RDF-aRat Dermal Fibroblasts-adult大鼠淋巴成纤维细胞R2530RLFRat Lymphatic Fibroblasts大鼠唾液腺成纤维细胞R2670RSGFRat Salivary Gland Fibroblasts大鼠肺肺泡上皮细胞R3200RPAEpiCRat Pulmonary Alveolar Epithelial Cells大鼠肺成纤维细胞R3300RPFRat Pulmonary Fibroblasts大鼠肾小管上皮细胞R4100RRPTEpiCRat Renal Proximal Tubular Epithelial Cells大鼠肾系膜细胞R4200RRMCRat Renal Mesangial Cells大鼠肝星状细胞R5300RHSteCRat Hepatic Slate Cells大鼠肝巨噬细胞R5340RHMaRat Hepatic Macrophage大鼠主动脉平滑肌细胞R6110RASMCRat Aortic Smooth Muscle Cells大鼠心肌细胞R6200RCMRat Cardiac Myocytes大鼠心肌成纤维细胞R6300RCFRat Cardiac Fibroblasts大鼠角膜基质细胞R6520RKRat Keratocytes大鼠晶状体上皮细胞R6550RLEpiCRat Lens Epithelial Cells大鼠间充质干细胞-骨髓R7500RMSC-bmRat Mesenchymal Stem Cells-bone marrow大鼠胚胎成纤维细胞R7540REFRat Embryonic Fibroblasts
(三)小鼠正常细胞
中文名称货号简称英文名称小鼠脑膜细胞M1400-57MMCMouse Meningeal Cells小鼠脑脊神经元M1510-57MN-rMouse Neurons-raphe小鼠皮质神经元M1520-57MN-cMouse Neurons-cortical小鼠颗粒细胞M1530-57MGCMouse Granule Cells小鼠海马趾神经元M1540-57MN-hMouse Neurons-hippocampal小鼠腹脊髓神经元M1570-57MN-vscMouse Neurons-ventral spinal cord小鼠背脊髓神经元M1580-57MN-dsc]Mouse Neurons-dorsal spinal cord小鼠腹脊髓神经元M1590-57MN-vscMouse Neurons-ventral spinal cord小鼠雪旺细胞M1700-57MSCMouse Schwann Cells小鼠星形胶质细胞M1800-57MAMouse Astrocytes小鼠小脑星形胶质细胞M1810-57MA-cMouse Astrocytes-cerebellar小鼠海马趾星形胶质细胞M1820-57MA-hMouse Astrocytes-hippocampal小鼠脊髓星形胶质细胞M1830-57MA-sc Mouse Astrocytes-spinal cord小鼠小胶质细胞M1900-57MMMouse Microglia小鼠巨噬细胞M1920-57MMa-bmMouse Macrophage小鼠淋巴成纤维细胞M2530-57MLFMouse Lymphatic Fibroblasts小鼠唾腺成纤维细胞M2670-57MSGF Mouse Salivary Gland Fibroblasts小鼠肾小管上皮细胞M4100-57MRTEpiCMouse Renal Tubular Epithelial Cells小鼠心肌细胞M6200-57MCMMouse Cardiac Myocytes小鼠心脏成纤维细胞M6300-57MCFMouse Cardiac Fibroblasts小鼠骨髓间充质干细胞M7500-57MMSC-bmMouse Mesenchymal Stem Cells-bone marrow小鼠胚胎成纤维细胞M7540-57MEF-mtMouse Embryonic Fibroblasts(四)猪细胞系统猪视网膜星形胶质细胞P1870PRAPorcine Retinal Astrocytes 猪角膜上皮细胞P6510PCEpiCPorcine Corneal Epithelial Cells猪视网膜色素上皮细胞P6540PRPEpiCPorcine Retinal Pigment Epithelial Cells 猪晶状体上皮细胞P6550PLEpiCPorcine Lens Epithelial Cells (五)马和狗细胞系统
马间充质干细胞--脂肪H7510HMSC-adHorse Mesenchymal Stem Cells-adipose马间充质干细胞--脂肪E7510EMSC-adEquine Mesenchymal Stem Cells-adipose狗间充质干细胞--脂肪D7510DMSC-adDog Mesenchymal Stem Cells-adipose
广州硕恒生物科技有限公司欢迎各位新老客户前来指导和洽谈合作!期待您的光临!
:
: